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KMID : 0861020100250010083
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Korea Journal of Herbology
2010 Volume.25 No. 1 p.83 ~ p.91
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Comparative Study of Anti-oxidant and Anti-inflammatory Activities between Curcumae Longae Radix and Curcumae Longae Rhizoma
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Oh Hye-In
Park Han-Byeol
Ju Mi-Sun
Chung Sun-Yong
Oh Myung-Sook
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Abstract
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Objectives: In this study, we compared the anti-oxidant and anti-inflammatory activities of Curcumae longae Radix (CLRa) and Curcumae longae Rhizoma (CLRh).
Methods: We performed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2-azinobis-3-ethylbenzothiazoline-
6-sulfonic acid (ABTS) cation scavenging assays, and determined total polyphenolic content to examine the anti-oxidant effects of CLRa and CLRh. We also evaluated the anti-oxidant effects of CLRa and CLRh against hydrogen peroxide (H2O2)-induced toxicity in PC12 cells using thiazolyl blue tetrazolium bromide (MTT) and reactive oxygen species (ROS) assays. Next, to compare the anti-inflammatory effects of CLRa and CLRh against lipopolysaccharide (LPS)-induced
inflammation in microglia BV2 cells, we measured nitric oxide (NO) assay and inducible nitrite synthase (iNOS) using Western blotting analysis.
Results: CLRa showed higher activity in DPPH and ABTS assays and lower total polyphenolic contents compared with CLRh. In PC12 cells, CLRa and CLRh showed no difference in H2O2-induced cell toxicity and ROS overproduction. In BV2 cells, CLRa showed higher effect than CLRh in NO and iNOS production induced by LPS.
Conclusions: These results demonstrate that CLRa has higher radical scavenging activities and anti-inflammatory effect in BV2 cells comparing CLRh. However, CLRa and CLRh have no effect and no difference in H2O2-induced toxicity.
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KEYWORD
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Curcumae Longae Radix, Curcumae Longae Rhizoma, anti-oxidant, anti-inflammatory, hydrogen peroxide, Lipopolysaccharide
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